While on the topic of Isolation, if we're to assume that the game is canon, that puts it five years prior to Romulus beginning. In Romulus, Weyland-Yutani clearly has access to Alien specimens on board the Renaissance Station, and 37 years later in Aliens the company doesn't know where exactly to look on LV-426 until Ripley is awakened and points them in the specific direction, leading to Burke sending the Jordan family off on that path.
Given the Derelict's signal being turned off in Isolation, and then Renaissance Station's samples presumably all being lost by the end of Romulus, it makes sense that the company, now without their hands on any samples again, would go back to prioritizing LV-426 as their main hope to acquire new samples, though without the signal active any more, they couldn't pinpoint the location of the Derelict directly as the Nostromo had been able to do back in Alien.